As of January 6, 2025, the LAS Pronto cluster was merged into the campus-wide Nova cluster. Information here may or may not work on Nova. Please contact Research IT if you have questions.

Blat

Parameters setting

For a large genome such as a human, corn, or soybean, it is better to run the parallel way to align the input sequence against each chromosomes separately.

You may see the error message as following for a large genome like human if you blast your sequence against the whole genome in one file:

Segmentation fault (core dumped)

It has nothing to do with the installation, as the software is designed for the small genome comparison.

Reference